General method of preparation of uniformly 13C, 15N-labeled DNA fragments for NMR analysis of DNA structures
Identifieur interne : 000197 ( France/Analysis ); précédent : 000196; suivant : 000198General method of preparation of uniformly 13C, 15N-labeled DNA fragments for NMR analysis of DNA structures
Auteurs : Brigitte René [France] ; Grégoire Masliah [France] ; Loussiné Zargarian [France] ; Olivier Mauffret [France] ; Serge Fermandjian [France]Source :
- Journal of Biomolecular NMR [ 0925-2738 ] ; 2006-11-01.
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- topic : ADN.
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Abstract
Summary: 13C, 15N labeling of biomolecules allows easier assignments of NMR resonances and provides a larger number of NMR parameters, which greatly improves the quality of DNA structures. However, there is no general DNA-labeling procedure, like those employed for proteins and RNAs. Here, we describe a general and widely applicable approach designed for preparation of isotopically labeled DNA fragments that can be used for NMR studies. The procedure is based on the PCR amplification of oligonucleotides in the presence of labeled deoxynucleotides triphosphates. It allows great flexibility thanks to insertion of a short DNA sequence (linker) between two repeats of DNA sequence to study. Size and sequence of the linker are designed as to create restriction sites at the junctions with DNA of interest. DNA duplex with desired sequence and size is released upon enzymatic digestion of the PCR product. The suitability of the procedure is validated through the preparation of two biological relevant DNA fragments.
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DOI: 10.1007/s10858-006-9075-0
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ISTEX:B19A014E14A003E56CBEAC0A7A26F2FB6789199DLe document en format XML
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